Identification of human cytomegalovirus strain with immediate-early (IE) antigen-specific monoclonal antibody is prevented by point mutation in IE gene
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چکیده
منابع مشابه
An Early Immediate Early Protein IE-1–Specific T-Cell Polyfunctionality Is Associated With a Better Control of Cytomegalovirus Reactivation in Kidney Transplantation
To the Editor: Immune response quality is considered as a major factor in chronic viral infections. Cytomegalovirus (CMV) remains one of most frequent and deleterious viral infections in kidney transplantation. For the prevention of CMV disease, prophylactic or preemptive treatments are commonly proposed. Because of side effects, new methods for tailoring of anti-CMV treatments are needed to ad...
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Background and Aims: Human Cytomegalovirus (HCMV) is one of the life-threatening agents in immunosuppressed patients and congenitally infected neonates in the world. Mutations in UL27 were suggested to confer low- to high-grade Maribavir (MBV) resistance. As pUL27 R233S variation may involve in either MBV-resistance, we aimed to establish a method for identifying R233 coding sequence mutation...
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VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was clonedin a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leadersequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Theconstruct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of...
متن کاملIdentification and purification of a specific and immunogenic antigen of the laminated layer of the hydatid cyst and production of an antigen-specific monoclonal antibody.
Cystic echinococcosis (CE) is an infection caused by the larval stage of Echinococcus granulosus. This is widely distributed through Iran, where a variety of animals act as intermediate host. The immunogenic antigens (Ag) of different compartments of the hydatid cyst have been already determined. One of these compartments is the laminated layer (LL). We have extracted a protein with the MW of 2...
متن کاملTelencephalon-specific antigen identified by monoclonal antibody.
Immunohistochemical screening of monoclonal antibodies (mAbs) raised against fractions rich in the dendrodendritic synaptosomes of the rabbit olfactory bulb revealed that one of the mAbs (mAb 271A6) recognized a telencephalon-specific antigen or antigens. Thus, the stain with mAb 271A6 was observed throughout the gray matter of all regions of the neocortex, piriform cortex, hippocampus, striatu...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1994
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.32.5.1402-1405.1994